To transduce a zebra finch: interrogating behavioral mechanisms in a model system for speech.

The ability to alter neuronal gene expression, either to affect levels of endogenous molecules or to express exogenous ones, is a powerful tool for linking brain and behavior. Scientists continue to finesse genetic manipulation in mice. Yet mice do not exhibit every behavior of interest. For example, Mus musculus do not readily imitate sounds, a trait known as vocal learning and a feature of speech. In contrast, thousands of bird species exhibit this ability. The circuits and underlying molecular mechanisms appear similar between disparate avian orders and are shared with humans. An advantage of studying vocal learning birds is that the neurons dedicated to this trait are nested within the surrounding brain regions, providing anatomical targets for relating brain and behavior. In songbirds, these nuclei are known as the song control system. Molecular function can be interrogated in non-traditional model organisms by exploiting the ability of viruses to insert genetic material into neurons to drive expression of experimenter-defined genes. To date, the use of viruses in the song control system is limited. Here, we review prior successes and test additional viruses for their capacity to transduce basal ganglia song control neurons. These findings provide a roadmap for troubleshooting the use of viruses in animal champions of fascinating behaviors-nowhere better featured than at the 12th International Congress

A Sing-Song Way of Vocalizing: Generalization and Specificity in Language and Birdsong.

Spoken languages such as German are extremely discrete, whereas others such as Portuguese are melodic or "sing-song" wherein identifying a word relies on what comes before and after. Perhaps surprisingly, birdsong also exhibits specificity and generalization as articulated by Tian and Brainard (2017)

Genome-wide patterns of polymorphism in an inbred line of the African malaria mosquito Anopheles gambiae.

Anopheles gambiae is a major mosquito vector of malaria in Africa. Although increased use of insecticide-based vector control tools has decreased malaria transmission, elimination is likely to require novel genetic control strategies. It can be argued that the absence of an A. gambiae inbred line has slowed progress toward genetic vector control. In order to empower genetic studies and enable precise and reproducible experimentation, we set out to create an inbred line of this species. We found that amenability to inbreeding varied between populations of A. gambiae. After full-sib inbreeding for ten generations, we genotyped 112 individuals--56 saved prior to inbreeding and 56 collected after inbreeding--at a genome-wide panel of single nucleotide polymorphisms (SNPs). Although inbreeding dramatically reduced diversity across much of the genome, we discovered numerous, discrete genomic blocks that maintained high heterozygosity. For one large genomic region, we were able to definitively show that high diversity is due to the persistent polymorphism of a chromosomal inversion. Inbred lines in other eukaryotes often exhibit a qualitatively similar retention of polymorphism when typed at a small number of markers. Our whole-genome SNP data provide the first strong, empirical evidence supporting associative overdominance as the mechanism maintaining higher than expected diversity in inbred lines. Although creation of A. gambiae lines devoid of nearly all polymorphism may not be feasible, our results provide critical insights into how more fully isogenic lines can be created

Beyond Critical Period Learning: Striatal FoxP2 Affects the Active Maintenance of Learned Vocalizations in Adulthood.

In humans, mutations in the transcription factor forkhead box P2 (FOXP2) result in language disorders associated with altered striatal structure. Like speech, birdsong is learned through social interactions during maturational critical periods, and it relies on auditory feedback during initial learning and on-going maintenance. Hearing loss causes learned vocalizations to deteriorate in adult humans and songbirds. In the adult songbird brain, most FoxP2-enriched regions (e.g., cortex, thalamus) show a static expression level, but in the striatal song control nucleus, area X, FoxP2 is regulated by singing and social context: when juveniles and adults sing alone, its levels drop, and songs are more variable. When males sing to females, FoxP2 levels remain high, and songs are relatively stable: this "on-line" regulation implicates FoxP2 in ongoing vocal processes, but its role in the auditory-based maintenance of learned vocalization has not been examined. To test this, we overexpressed FoxP2 in both hearing and deafened adult zebra finches and assessed effects on song sung alone versus songs directed to females. In intact birds singing alone, no changes were detected between songs of males expressing FoxP2 or a GFP construct in area X, consistent with the marked stability of mature song in this species. In contrast, songs of males overexpressing FoxP2 became more variable and were less preferable to females, unlike responses to songs of GFP-expressing control males. In deafened birds, song deteriorated more rapidly following FoxP2 overexpression relative to GFP controls. Together, these experiments suggest that behavior-driven FoxP2 expression and auditory feedback interact to precisely maintain learned vocalizations

Improving Access to Books for Young School-age Children One Library at a Time

Johnson and Donham (2012) found that Iowa elementary school library check out policies for young children did not always support early literacy development because of their being overly restrictive concerning the number of books children could borrow each week. A state-wide survey of Idaho elementary school library staff revealed similar challenges. Preschoolers, kindergarteners, and 1st graders were not being given adequate access to books. A state-wide grant program was developed and implemented that provided funds for collection development while stipulating the implementation of less restrictive check out policies for young children. Results from the grant program are reported along with future plans and recommendations for others wanting to implement similar programs

The Role of GIS to Enable Public-Sector Decision Making Under Conditions of Uncertainty

Uncertainty is inherent in environmental planning and decision making. For example, water managers in arid regions are attuned to the uncertainty of water supply due to prolonged periods of drought. To contend with multiple sources and forms of uncertainty, resource managers implement strategies and tools to aid in the exploration and interpretation of data and scenarios. Various GIS capabilities, such as statistical analysis, modeling and visualization are available to decision makers who face the challenge of making decisions under conditions of deep uncertainty. While significant research has lead to the inclusion and representation of uncertainty in GIS, existing GIS literature does not address how decision makers implement and utilize GIS as an assistive technology to contend with deep uncertainty. We address this gap through a case study of water managers in the Phoenix Metropolitan Area, examining how they engage with GIS in making decisions and coping with uncertainty. Findings of a qualitative analysis of water mangers reveal the need to distinguish between implicit and explicit uncertainty. Implicit uncertainty is linked to the decision-making process, and while understood, it is not displayed or revealed separately from the data. In contrast, explicit uncertainty is conceived as separate from the process and is something that can be described or displayed. Developed from twelve interviews with Phoenix-area water managers in 2005, these distinctions of uncertainty clarify the use of GIS in decision making. Findings show that managers use the products of GIS for exploring uncertainty (e.g., cartographic products). Uncertainty visualization emerged as a current practice, but definitions of what constitutes such visualizations were not consistent across decision makers. Additionally, uncertainty was a common and even sometimes helpful element of decision making; rather than being a hindrance, it is seen as an essential component of the process. These findings contradict prior research relating to uncertainty visualization where decision makers often express discomfort with the presence of uncertainty.

Librarians: Key Partners in a State-Wide Book Distribution Outreach Program

Book distribution programs targeted at young children and their families that include the children own-ing the books have been in existence for a long time. Results are provided from a multi-year program evaluation of a unique, state-wide book distribution program developed in 1997 by the Idaho Commis-sion for Libraries called My First Books. The program is unique among book distribution programs because it relies on local librarians to deliver and showcase the books each month during the academic year in a variety of public and private early childhood care and education settings. My First Books thus becomes a powerful mechanism for library outreach to local childcare and education facilities, parents and caregivers of young children, and the children themselves. Program evaluation results reveal a highly popular and effective program for all of these constituencies that fits well within the resources available in public libraries of all sizes throughout Idaho

Striatal FoxP2 Is Actively Regulated during Songbird Sensorimotor Learning

BACKGROUND: Mutations in the FOXP2 transcription factor lead to language disorders with developmental onset. Accompanying structural abnormalities in cortico-striatal circuitry indicate that at least a portion of the behavioral phenotype is due to organizational deficits. We previously found parallel FoxP2 expression patterns in human and songbird cortico/pallio-striatal circuits important for learned vocalizations, suggesting that FoxP2's function in birdsong may generalize to speech. METHODOLOGY/PRINCIPAL FINDINGS: We used zebra finches to address the question of whether FoxP2 is additionally important in the post-organizational function of these circuits. In both humans and songbirds, vocal learning depends on auditory guidance to achieve and maintain optimal vocal output. We tested whether deafening prior to or during the sensorimotor phase of song learning disrupted FoxP2 expression in song circuitry. As expected, the songs of deafened juveniles were abnormal, however basal FoxP2 levels were unaffected. In contrast, when hearing or deaf juveniles sang for two hours in the morning, FoxP2 was acutely down-regulated in the striatal song nucleus, area X. The extent of down-regulation was similar between hearing and deaf birds. Interestingly, levels of FoxP2 and singing were correlated only in hearing birds. CONCLUSIONS/SIGNIFICANCE: Hearing appears to link FoxP2 levels to the amount of vocal practice. As juvenile birds spent more time practicing than did adults, their FoxP2 levels are likely to be low more often. Behaviorally-driven reductions in the mRNA encoding this transcription factor could ultimately affect downstream molecules that function in vocal exploration, especially during sensorimotor learning

Enhanced Storytimes: Effects on Parent/Caregiver Knowledge, Motivation, and Behaviors

The article offers information regarding the released of the Every Child Ready @ your library initiative\u27s second edition of the Public Library Association and the Association for Library Service to Children in 2011. It states that the initiative features five practices based in high-quality oral language development in children such as reading, writing, and talking. It mentions that the initiative will help children in early literacy development and will educate caregivers and parents

FoxP2 isoforms delineate spatiotemporal transcriptional networks for vocal learning in the zebra finch.

Human speech is one of the few examples of vocal learning among mammals yet ~half of avian species exhibit this ability. Its neurogenetic basis is largely unknown beyond a shared requirement for FoxP2 in both humans and zebra finches. We manipulated FoxP2 isoforms in Area X, a song-specific region of the avian striatopallidum analogous to human anterior striatum, during a critical period for song development. We delineate, for the first time, unique contributions of each isoform to vocal learning. Weighted gene coexpression network analysis of RNA-seq data revealed gene modules correlated to singing, learning, or vocal variability. Coexpression related to singing was found in juvenile and adult Area X whereas coexpression correlated to learning was unique to juveniles. The confluence of learning and singing coexpression in juvenile Area X may underscore molecular processes that drive vocal learning in young zebra finches and, by analogy, humans